Composite
TRT

Part:BBa_K3262000:Design

Designed by: Shivsam Bady, Surabhi Singh   Group: iGEM19_SNU_India   (2019-10-14)


pT7+RFP+TAL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 797
    Illegal NgoMIV site found at 1629
    Illegal AgeI site found at 593
    Illegal AgeI site found at 705
    Illegal AgeI site found at 892
    Illegal AgeI site found at 1058
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2087


Design Notes

Part was cloned in pSB1C3 and pSB3C5 (low copy number plasmid)


Source

DNA Synthesis

References